Assessment of the Patient with Acute Abdominal Pain Article

Assessment of the Patient with Acute Abdominal Pain - Article Example Assessment by the use of a thorough history can determine the cause of up to seventy percent of all abdominal pain cases. The history plan laid out by the authors questions involving the pain itself, what symptoms are co-morbid with the pain, past medical treatment, current medications and medical conditions, and a family and social history. Article Evaluation The guide for the history laid out in this article is very detailed. It even gives notes on how to frame the questions in order to gain the most useful results from the patient, as well as listing nearly a dozen co-morbid disorders that can lead to abdominal pain during serious complications. It also provides an explanation of many of the therapies that are often used to treat and diagnosis abdominal pain. However, most of the diagnostic tools mentioned and all of the treatment methods would require a physician and are outside the scope of traditional nursing care. Despite this, there are no reasons why this assesssment should not be used.

Gillette Companys Marketing Plan Essay Example | Topics and Well Written Essays - 1500 words

Gillette Companys Marketing Plan - Essay Example Gillette is such one brand of this corporation. It is a brand of safety razors and other personal hygiene range of products. The razors of this company protect the skin from all parts of the razor except the edge alone. The skin is in contact with the edge of the razor alone. This is as opposed to cut-throat razors also called straight razors. Here, the skin is contact with the whole of the razor. A safety razor reduces the risk of injury to the skin. That is the reason why they are preferred by men for shaving. Gillette used to be a company on its own. It was started in 1895 (Bonoma 56). The founder was King C. Gillette. He named the company after himself. It used to deal with a range of consumer goods, the safety razors included. However, in the year 2005, more than a century after been founded, the company was acquired by Procter & Gamble (Bonoma 57). That is how it ended up been a brand of this multinational corporation. It is headquartered in Boston, Massachusetts. It no longer exists as a company now, but as a brand. Not only does Gillette produce safety razors for shaving, it also has a range of other products. It produces antiperspirants, hair care and body products. Its products are aimed for grooming, especially in males. It has Gillette shave gel and after shave (Cundiff 98). These products are designed to make the shaving experience for the man as comfortable as possible. Gillette's anti-deodorant is said to be of clinical status. The hair products are also designed for the man (Bonoma 25). They include stylers, shampoos and hair conditioners. It also has a wide range of shower gels for men (Ferrell 56). They include those aimed for dry skin, oily and normal skin. They also include face wash gels for men. The gels were hitherto thought to be the preserve of women. Target Market for Gillette Products The target market for Gillette Company products is the young men aged between sixteen and thirty-four years (Cundiff 36). The grooming products especially are targeted at this age bracket. Gillette has aggressive promotional campaigns aimed at this group. The challenge is to convince these men that grooming is not the preserve of women. It used to sponsor a soccer game every Saturday in the 1990's. This was called Soccer Saturday. It was aired by Sky Sports Channel (Ferrell 98). An average of one million young males watched this program in the year 2005-2006. This was an average of one million every month. This made up around a quarter of the target population for the Gillette products (Bonoma 98). Gillette has used Soccer Saturday to not only market the existing brands, but also as a launching pad for new products (Ferrell 78). This is what happened when the company introduced Mach3, Turbo and M3Power range of products. In terms of market growth, the company's products continue to enjoy popularity amongst the target population. They are the most famous. Gillette is likely to remain the leader in this market. A SWOT Analysis of Gillette Company Strengths Gillette Company has obvious strengths that work to its advantage. These are internal factors that help it to remain the most popular company in this area. First, the company is managed by qualified professionals (Cundiff 45). The staffs are also highly qualified professionals in their field. For a company to be productive, every sector must be headed by a qualified person. Every department in this company is headed by a quali

Ethical Implications for Human Trafficking in the United States Essay Example for Free

Ethical Implications for Human Trafficking in the United States Essay Ethical Implications for Human Trafficking in the United States Human trafficking is a type of modern-day slavery in which millions of people, regardless of gender or age, around the world are forced into. Human trafficking is the trade of human beings for the purpose of forced labor, sexual exploitation or illegal profits and usually involves the use of violence, fraud, or coercion to recruit, hide, and transport people illegally (Act Now). According to the United Nations is both a definition of human trafficking is â€Å"The recruitment, transportation, transfer, harboring or receipt of persons, by means of the threat or use of force or other forms of coercion, of abduction, of fraud, of deception, of the abuse of power or of a position of vulnerability or of the giving or receiving of payments or benefits to achieve the consent of a person having control over another person, for the purpose of exploitation. Exploitation shall include, at a minimum, the exploitation of the prostitution of others or other forms of sexual exploitation, forced labor or services, slavery or practices similar to slavery, servitude, or the removal of organs. † (UNICEF) The traffickers use different types of strategies to exploit the â€Å"slaves†. There are two types of slavery: those who are forced to work for money and those who are kidnapped and forced to work for food (â€Å"Human Trafficking†). Trafficking primary involves exploitation which comes in many forms such as forcing victims into prostitution, subjecting victims to slavery or involuntary servitude, compelling victims to commit sexual acts for the purpose of creating pornography and misleading victims into debt bondage (Do Something). Of the 27 million slaves around the world, 80% of trafficking involves sexual exploitation, and 19% involves labor exploitation (Do Something). Many of the people who are victims of human trafficking are people who voluntarily enter the business but under false pretenses. These people leave their homes in order to change their quality of life but many of the times they are lied to about the conditions of the work they are intended to do. Due to monetary problems or joblessness, people try to find better opportunities out of the country. Poverty is a huge contributor to human trafficking as well. Mostly in third world countries and sometimes in second world counties, family members will sell females and children for cash. Females can cost around US$100-2,500 depending on the country, the age of the female and if she has had a child or not (PBS). Then they must work off the debt they accumulate, which includes costs of food, shelter, and other expenses with interest which can take years to settle. Homeless children or children who come from broken families are more susceptible to the sex industry to survive economically. These innocent victims put their trust on â€Å"manager† or the â€Å"middle man† who promise them a higher paying job in a better area. Many women leave their homes in order to find better opportunities for a high paying job for their family but when they arrive to the new city, they are forced into an exploitative job. They way human traffickers secure the â€Å"slaves† is by making them dependant on them by taking their passport away, making them into drug addicts and with emotional and physical abuse. Although 110 countries have signed and approved the document, implanting the policies and law proves most difficult. The challenge lies in targeting all the criminals who target innocent and vulnerable people who organize the human trafficking ring. In March 2007, the United Nations Office on Drugs and Crimes (UNODC) formally launched a Global Initiative to fight Human Trafficking meaning they will help draft laws and help implement policies against human trafficking. The biggest international milestone to date has been the adoption of the Protocol to Prevent, Suppress, and Punish Trafficking in Persons, Especially Women, and Children (Act Now). The reason why it is so difficult to eradicate human trafficking is due to economical reasons. In economic terms, by increasing the amount of product in the most cost effect manner, the cheaper the product which is favorable for all consumers. However, putting it in simpler worlds, to provide lower prices for consumers, human traffickers reduce the cost of production by providing cheap labor to the factories. In order to find a better opportunity for their life, many people are being trafficked voluntarily. This means that they sign an â€Å"indentured servitude† agreement in which they are smuggled from one country into another. Even though this is illegal and exploitative, it allows people to move to a place that might have more resources to sustain them, which could balance the burden of human population. Because human trafficking allows some companies to produce goods and services at a lower cost, the other companies that compete legally will have to innovate and improve their businesses to stay competitive. Meaning human trafficking encourages the development of more efficient and innovative technology by providing cheap labor. Human trafficking dehumanizes the victims and rewards the perpetrators. With 50% of all slaves are under the age of 18 years, two thirds of them suffer from physical abuse from their handlers and it affects them in the long run (Do Something). They are more likely to develop metal health problems, engage in substance abuse, engage in prostitution and either commit, or be a victim of violent crimes (Do Something). We may think that things like this could never occur where we live but Orlando is the perfect example of how human trafficking in everywhere. According to Giselle Fernandez of the Coalition Against Human Trafficking, Florida is the number two destination for human trafficking due to the theme parks. Many of the girls work at the hotels, become prostitutes or end up in pornography. In order to prevent such a heinous crime, females must be educated so they are less susceptible to false hopes. Increasing police pay in certain destination areas so they are less likely to be bribed by traffickers. Only with efforts from the government, private companies, and especially communities will we be able to eradicate human trafficking.

Anti-tumour Immunity through GP-100-TLR Agonist Conjugation

Anti-tumour Immunity through GP-100-TLR Agonist Conjugation Enhancing anti-tumour immunity through gp-100-TLR agonist conjugation. Introduction Soluble cancer vaccines remain an area of high interest to researchers with the ability to enhance immune responses against present cancers and induce protective immunity against future cases. In developing new vaccines finding ways to increase the immunogenicity of cancer antigens is a major challenge(1-3). The addition of Toll-like receptor (TLR) agonists is one strategy which can successfully boost immune cell activation and response to cancer antigens. By stimulating TLRs, these agonists increase expression of several co-stimulatory molecules on antigen presenting cells (APCs) such as CD80/86 and CD40(4-6).They also increase tumour peptide loading onto type 1 2 Major histocompatibility complex (MHC) proteins. Together this leads to greater activation of tumour-specific effector immune cells such as CD4+ and CD8+ T-cells resulting in increased tumour clearance via their cytotoxic activity. Vaccine formulations which have included antigen and TLR agonists as a mixture have had pro mising results with many in clinical trials(4, 7, 8). Despite this, few have assessed the effect of chemically conjugating these constituents, a strategy which could increase efficiency of both TLR activation and peptide loading onto MHC(9-12). Many conjugation strategies that do exist today capitalise on the use of pH and redox sensitive linkers. Differences in pH and redox environments intracellularly enable triggerable release of these vaccines whilst protecting antigen and agonist from degradation extracellularly where they are administered. Research into the use of Glutathione-sensitive disulphide linkers has demonstrated that the immune response to model antigen Ovalbumin'(OVA) could be increased through linkage to the TLR agonist, CpG oligodeoxynucleotide (ODN)(10, 11). Our research aims to repeat this using both stable and reversible linkers as well as a more clinically relevant, tumour associated antigen (TAA) called gp-100 expressed on melanomas. In addition, we aim to ass ess the effectivity of different TLR agonists within conjugates including Polyinosinic polycytidylic acid (Poly I:C) and two different classes of CpG ODNs, B and C respectively. Each of these agonists activate different signalling pathways within antigen presenting cells leading to unique cytokine profiles and T-cell responses. Poly I:C for example, is a potent activator of TLR3 which activates the TRIF pathway inducing release of type 1 interferons such as IFN Beta(6, 13, 14). This increases MHC-I expression and stimulates a Th1 type immune response which favours cell-mediated immunity including CD8+ T-cell activation. In comparison, CpG class B and C stimulate TLR 9 activating the MYD88 pathway and release of proinflammatory cytokines IL-6 and IL-17. This results in enhanced CD4+ and CD8+ T-cell responses, B cell activation and antibody production(10, 11, 15). Both types of response have potential to give clinical benefit in different ways highlighting the potential of these conju gates in tumour treatment. Finally, we will also assess how the composition of the TAA effects its presentation on MHC. To assess this, a smaller Gp-100 peptide which does not require intracellular processing will be compared to a longer peptide requiring processing. This project will assess which conjugates enhance anti-tumour responses in mice and how they achieve this looking specifically at Dendritic cell activation and CD8+ T-cell proliferation and cytokine production. Hypotheses In terms of CD8+ responses, T cell proliferation and cytokine release, I hypothesize that Poly I:C reversibly linked to processed gp-100 will be the most effective inducing a strong Th1 response and IFN-B cytokine release. This is because Poly I:C stimulates several intracellular signals in addition to TLR3 including RIG-1 and MDA-5. This would lead to increased release of proinflammatory cytokines including IL-6, IL-12, IL-1B and IFN-B. Specifically the release of IFN-B would induce a strong anti-viral-like Th1 T cell response through increased expression of MHC-1 molecules on APCs and inducing release of IFN-Y, TNF-A and IL-2 from CD4+ T cells. As well as this factor, TLR3 is exclusively expressed on myeloid dendritic cells, the most effective dendritic cell subset in presenting antigen, and not expressed on plasmacytoid dendritic cells. Secondly, I Hypothesize that CpG class C will induce the most cytokine production in dendritic cells including the cytokines IL-6, IL-12, IFN-A and IL-1B. CpG molecules stimulate TLR9 which is expressed in the endolysosomal compartment of plasmacytoid dendritic cells exclusively. As a dendritic cell subset, plasmacytoid DCs are well known for their proinflammatory cytokine production at levels much higher than other DC subsets. CpG class C in particular stimulates the release of IFN-a in addition to IL-6, IL-12 etc. stimulating both a Th1 response and a B cell response. Aims and objectives Aims Produce gp-100-CpG ODN and gp-100-Poly I:C reversible and stable conjugates with either processed or non-processed Gp-100 peptides Measure dendritic cell subset activation through expression of MHC-II, CD40 and CD86 molecules and cytokine release (IL-12, IL-6, IL-1B, IFN-A and IFN-B ) Measure Tumour specific T cell activation (CD4+ and CD8+), proliferation using carboxyfluorecein succinimidyl ester (CSFE) and cytokine release (IFN-Y, IL-2,TNF-A) Methods The proposed project for the year will focus on three main objectives 1) Produce gp-100-CpG ODN and gp-100-Poly I:C reversible and stable conjugates with either processed or non-processed Gp-100 peptides. First, we will modify free amino groups on the lysine residues of each gp-100 peptide (processed amino acid sequence: KVPRNQDWL vs unprocessed: CAVGALKVPRNQDWLGVPRQL) and TLR agonists (suspended in a modification buffer ph. 8). Then we will link these together with either the stable linker (HYN) or the reversible linker (HYN-SS) in a ph. 6 conjugation buffer separately. Product concentration after each individual modification step will be measured using Nanodrop1000 at 280 m after desalting excess product using vivspin 500 filter. Final product conjugation will be confirmed using the reversed phase liquid chromatograph at the School of Pharmacy which will allow us to visualise each individual product according to their differing polarities, and quantify their ratio. Our second objective is to Measure dendritic cell subset activation through expression of MHC-II, CD40 and CD86 molecules and cytokine release (IL-12, IL-6, IL-1B, IFN-B, IFN-A). To achieve this, we will isolate bone marrow cells from C57BL/6 mice and treat with GM-CSF to produce CD11c+ dendritic cells. These will then be treated with either individual TLR agonists, TLR agonist-gp100 mixtures or TLR agonist-gp100 conjugates (reversible or non-reversible). After 24hrs of treatment these cells with be stained with fluorescent antibodies for CD80, CD40, CD11C, and MHC-II, viewed on the Gallios flow cytometer in Pathology and analysed using Kaluza software. This experiment will be repeated at least three times to enable statistical analysis, which will be performed using Graph Pad prism software. Cytokine release from these cells will be measured using an enzyme linked immunosorbent assay (ELISA) for IL-6, IL-12, IFN-B and IFN-a. Our third objective is to Measure Tumour specific CD3+ T cell: activation (CD8+), proliferation (CSFE) and cytokine release (IFN-Y, IL-2). This will be achieved through isolation of splenocytes from Pmel (T-cells specific to gp-100) transgenic mice and sorting of CD8+ cells using the Automacs machine at Pathology. These cells will then be stained using CSFE and co-cultured separately with C57BL/6 BMDCs treated according to objective 2. After 72hrs cells will be analysed using the Gallios flow cytometer to measure T-cell activation (CD3+) and proliferation (CSFE). To measure cytokine release, cell cultures will undergo an ELISA for IFN-Y and IL-2. Proposed Budget Mice C57BL/6 x 10 @ $50 each$500 PMEL x 10 @ $50 each$500 Antibodies CD86-PE$300 CD11c-APC$300 CD40-PECy7$300 CD8a-APC$300 CD3-PE$300 MHC-I$300 MHC-II FITC$300 Cell culture reagents IMDM Media$400 Foetal calf serum$500 Cytokine detection Cytokine detection kit$2000 Conjugation reagents S4FB Linker$450 S-SS-4FB Linker$350 S-HYNIC cross linker$850 2-Hydrazinopyradine.dihydrochloride$450 2-Sulphobenzaldehyde$450 CpG class B$500 CpG class C$500 Poly I:C$500 Vivspin filters$200 Total$9250 References 1.Obeid JM, Hu Y, Slingluff CL. Vaccines, adjuvants and dendritic cell activators Current Status and Future Challenges. Seminars in oncology. 2015;42(4):549-61. 2.Guo C, Manjili MH, Subjeck JR, Sarkar D, Fisher PB, Wang X-Y. Therapeutic Cancer Vaccines: Past, Present and Future. Advances in cancer research. 2013;119:421-75. 3.Schlom J. Therapeutic Cancer Vaccines: Current Status and Moving Forward. JNCI Journal of the National Cancer Institute. 2012;104(8):599-613. 4.Kaczanowska S, Joseph AM, Davila E. TLR agonists: our best frenemy in cancer immunotherapy. Journal of leukocyte biology. 2013;93(6):847-63. 5.Pradere J-P, Dapito DH, Schwabe RF. The Yin and Yang of Toll-like Receptors in Cancer. Oncogene. 2014;33(27):3485-95. 6.Maruyama K, Selmani Z, Ishii H, Yamaguchi K. Innate immunity and cancer therapy. International immunopharmacology. 2011;11(3):350-7. 7.Iribarren K, Bloy N, Buque A, Cremer I, Eggermont A, Fridman WH, et al. Trial Watch: Immunostimulation with Toll-like receptor agonists in cancer therapy. Oncoimmunology. 2016;5(3):e1088631. 8.Dowling JK, Mansell A. Toll-like receptors: the swiss army knife of immunity and vaccine development. Clinical Translational Immunology. 2016;5(5):e85. 9.Flanary S, Hoffman AS, Stayton PS. Antigen delivery with poly(propylacrylic acid) conjugation enhances MHC-1 presentation and T-cell activation. Bioconjugate chemistry. 2009;20(2):241-8. 10.Herbath M, Szekeres Z, Kovesdi D, Papp K, Erdei A, Prechl J. Coadministration of antigen-conjugated and free CpG: effects of in vitro and in vivo interactions in a murine model. Immunology letters. 2014;160(2):178-85. 11.Kramer K, Shields NJ, Poppe V, Young SL, Walker GF. Intracellular Cleavable CpG Oligodeoxynucleotide-Antigen Conjugate Enhances Anti-tumor Immunity. Molecular Therapy. 2017;25(1):62-70. 12.Slutter B, Soema PC, Ding Z, Verheul R, Hennink W, Jiskoot W. Conjugation of ovalbumin to trimethyl chitosan improves immunogenicity of the antigen. Journal of controlled release : official journal of the Controlled Release Society. 2010;143(2):207-14. 13.Ammi R, De Waele J, Willemen Y, Van Brussel I, Schrijvers DM, Lion E, et al. Poly(I:C) as cancer vaccine adjuvant: knocking on the door of medical breakthroughs. Pharmacology therapeutics. 2015;146:120-31. 14.Cho HI, Barrios K, Lee YR, Linowski AK, Celis E. BiVax: a peptide/poly-IC subunit vaccine that mimics an acute infection elicits vast and effective anti-tumor CD8 T-cell responses. Cancer immunology, immunotherapy : CII. 2013;62(4):787-99. 15.Scheiermann J, Klinman DM. Clinical evaluation of CpG oligonucleotides as adjuvants for vaccines targeting infectious diseases and cancer. Vaccine. 2014;32(48):6377-89.

Ideas as Things Essay -- Metaphors Essays

There are many metaphors used to illustrate the process of learning or understanding. Educational theorists use metaphors to describe and demonstrate their theories, but ordinary people also use metaphors to describe cognition. For example, we use images of light to express thought; we have ‘‘bright ideas’’ or ‘‘dark thoughts,’’ or see we the ‘‘light of reason.’’ Atmospheric conditions are also used to describe the quality of someone’s thinking, a person can be an ‘‘airhead,’’ plans can be hazy, and memory can cloud. The common metaphors used to describe learning and cognition differ from those used by modern educational theorists. Most modern thinkers in educational psychology use the metaphor of thoughts and memories as objects with real locations in space. In their models of thought and cognition, ideas and memories can be organized, built with, be used to create frameworks with; they can even become rigid and patterned. In addition, if our thoughts do become rigid we can ‘‘soften’’ them by moving laterally rather than building further on the structures we have already created. In the modern educational philosopher’s paradigm, ideas are no longer the ephemeral products of light and air. Instead, they have become concrete objects that take up space. Ideas, for psychologists, are ‘‘things’’ that we can manipulate. Can I touch this? In spite of the current popularity of the paradigm that thoughts are physical, the conception of thoughts as light or air, as noted above, persists in common usage. When we say that someone has a bright idea, we do not mean that the idea actually illuminates anything. Instead, we mean that his or her idea makes sense to us. It is an interesting metaphor because it likens understandin... ...e ancient epistemological debate still rages. At least ordinary people have not decided whether to allow for the existence of an immaterial mind, whose action is not completely dependent upon the mechanical functioning of the physical brain. Cognitive psychologists, perhaps, believe that thoughts are matter, but common metaphors hint that regular people are not yet wholly convinced. Works Cited The American Heritage Dictionary of the English Language. 4th ed. : Houghton Mifflin, 2000. Glover, John A., Royce R. Ronning, and Cecil R. Reynolds. Handbook of Creativity. 1st ed. : Springer, 1989. Harper, Douglas. Online Etymology Dictionary . Nov 2002. 23 Sep. 2005 . Kearsley, Greg. ‘‘TIP: The Theories.’’ Theory into Practice. 14 Sep. 2005 . gOFFICE.com 3 Ideas as Things Essay -- Metaphors Essays There are many metaphors used to illustrate the process of learning or understanding. Educational theorists use metaphors to describe and demonstrate their theories, but ordinary people also use metaphors to describe cognition. For example, we use images of light to express thought; we have ‘‘bright ideas’’ or ‘‘dark thoughts,’’ or see we the ‘‘light of reason.’’ Atmospheric conditions are also used to describe the quality of someone’s thinking, a person can be an ‘‘airhead,’’ plans can be hazy, and memory can cloud. The common metaphors used to describe learning and cognition differ from those used by modern educational theorists. Most modern thinkers in educational psychology use the metaphor of thoughts and memories as objects with real locations in space. In their models of thought and cognition, ideas and memories can be organized, built with, be used to create frameworks with; they can even become rigid and patterned. In addition, if our thoughts do become rigid we can ‘‘soften’’ them by moving laterally rather than building further on the structures we have already created. In the modern educational philosopher’s paradigm, ideas are no longer the ephemeral products of light and air. Instead, they have become concrete objects that take up space. Ideas, for psychologists, are ‘‘things’’ that we can manipulate. Can I touch this? In spite of the current popularity of the paradigm that thoughts are physical, the conception of thoughts as light or air, as noted above, persists in common usage. When we say that someone has a bright idea, we do not mean that the idea actually illuminates anything. Instead, we mean that his or her idea makes sense to us. It is an interesting metaphor because it likens understandin... ...e ancient epistemological debate still rages. At least ordinary people have not decided whether to allow for the existence of an immaterial mind, whose action is not completely dependent upon the mechanical functioning of the physical brain. Cognitive psychologists, perhaps, believe that thoughts are matter, but common metaphors hint that regular people are not yet wholly convinced. Works Cited The American Heritage Dictionary of the English Language. 4th ed. : Houghton Mifflin, 2000. Glover, John A., Royce R. Ronning, and Cecil R. Reynolds. Handbook of Creativity. 1st ed. : Springer, 1989. Harper, Douglas. Online Etymology Dictionary . Nov 2002. 23 Sep. 2005 . Kearsley, Greg. ‘‘TIP: The Theories.’’ Theory into Practice. 14 Sep. 2005 . gOFFICE.com 3

Anne Frank Remembered: Review Essay example -- essays research papers

Anne Frank Remembered: Review Anne Frank Remembered is the autobiography of Miep Gies, the woman who helped the Frank family survive during their two years in hiding. Her book is a primary source or first hand account of the persecution of Jewish people in Nazi occupied Holland during the second world war. It is also the first hand account of the hiding of Jews such as the Frank family, the Van Daan family, and Dr. Albert Dussel during this time. In regard to the book's autobiographical format, the author, Miep Gies, does not present the reader with a clear thesis statement. Instead, throughout the book the author discusses her main views toward the actions of the Nazis and their oppression of the Jewish people. Her disapproval of German Nazi actions is evident in the following quotation, when she was asked to join the Nazi Girls' Club: " 'How can I join such a club?' I icily asked. 'Look at what the Germans are doing to the Jews in Germany.' ...Let her take a good look at me and see with her own eyes that some 'Aryan' woman was not to be swept in by the Nazis." (Gies, p. 41, 1987). The main source of background to the author's viewpoint is her own story. In order to further discuss her main points and views, a summary of her story must be given. The book began with a brief history of the childhood of Miep Gies. She was born in Vienna, Austria in 1909, where she lived with her parents until the age eleven year. She was then sent to Amsterdam by a program in the aid of undernourished and sick children and was to be adopted by a Dutch family. She became used to the Dutch way of life as she grew older and soon she began to consider herself Dutch, not Viennese. Her association with the Frank family began when she was given a job with the Pectacon Company, owned and operated by Mr. Otto Frank. His company made and sold pectin, which was used for making jam. Miep's first part of the job was to make jam with different formulas of pectin. After becoming an expert jam maker, she was placed at a desk in the office to do office work. She became very close to the Frank family and was invited to their home regularly for meals. She also began a relationship with a man named Jan, whom she later married. &... ...e Nazis were doing to people. It indicates an intended audience of most likely those who have already read The Diary of Anne Frank and are looking for further investigation on the topic. However, it is not required that the reader has read The Diary of Anne Frank before reading this book. Miep Gies starts from the very beginning of her association with the Frank family and completes the story of their life. Although this book is recommended to anyone who is interested in this topic, the book may also be directed towards those of Jewish decent who experienced similar instances and want to find out what happened to others. In final evaluation, I have found this book to be very convincing, as it is a true story. The reader is left at the end of the book to draw their own opinions on the topic and the author's account of the story. I found that the author's use of evidence in her book was very good because her main source was her own story as an eye witness, with pictures and copies of documents to prove that the information is true. The book is very useful in understanding the issue of the persecution of Jews during the Holocaust in the second world war.

Ethnographic Observation

I set out to find a place to begin my observations, not knowing what to fully expect, what I may find. So I decided to look around at what is close to my home that isn’t a place I frequent or have even visited at all. Then it came to me, the Starbucks that is only about a mile away is a perfect place for me to observe subjects that I would consider different from myself, seeing as how I consider such obscene prices for coffee ridiculous. Starbucks is a very popular chain of coffee vendors that describe their product as more about quality than what Americans are used to in typical coffee joints. Although I know it is poor technique of me as an anthropologist to have prejudice about any place or people that may be at that place, but I have to admit I already had set in my mind that these people would be â€Å"stuck-up† â€Å"more money than sense† type of people. So upon entering the establishment I decided to blend into as much as possible by ordering one of their Grande size coffees, which is just their basic coffee in a medium size. Then, I found a spot in the corner and began to pretend I was reading the newspaper I had brought as a prop to further help me blend into my surroundings. The aroma of the building was very pleasant blend of coffee and various mixtures of chocolate and hazelnut. It also did have a very friendly feel and accepting sense to the whole thing. So after being in Starbucks for approximately thirty minutes I decided I had enough data to discuss my findings. The business definitely drew in a particular social group. It seemed to consist of mainly white males and females that appeared to be in their early twenties to upper twenties. Also most of them seemed to be more affluent to at least upper middle-class status. I came to this conclusion by the vehicles they drove and also the clothing they were wearing. Most of them were adorned in such brands as Columbia and The North Face, which are higher-end winter apparel. Most of their vehicles appeared to be if not new only a couple of years of age and were in very nice condition. Most of the groupings were either just singular people or couples coming to pick up either a plain coffee or most of the females ordering some mixed espresso or something to that degree. There were a couple of oddities in the data such as a one older couple that came by and also one black male also stopped by to pick up some coffee. Most the behavior I observed was a very casual and relaxed as the people seemed to all be friendly and enjoying their absurd priced beverages. Once in awhile someone would recognize a fellow customer and they would proceed to great each other with either a handshake or hug. Spatial arrangement between people was typical of most American norms. They typically had about a person length in between each person making sure not to get to close to bump into one another which is considered rude in this country. I did my research at approximately 1pm to 1:30pm in the afternoon. I do believe this did influence the type of people I saw in the establishment. If it were early in the morning I believe there would have been slightly older gathering of people going to their respectable jobs. Instead a majority of the customers were college students that were coming in for that mid day pick-up. As for the norms I witnessed they were typical for American establishments, the people working greeted the customers in an almost fake friendly manner that is supposed to comfort and make the customer feel â€Å"at home† in the environment. Then, the customer would reply with what they would like to order and then finish by paying for the product, in this case coffee. In conclusion I have to say that for the most part my presumptions were reinforced which is consistent with what I figured would be the case. Although, it isn’t entirely fair because I didn’t get to truly meet my subjects and understand them fully. I only observed how they behaved in that instance which may have been completely out there norm and even their first time at Starbucks.